Coding

Part:BBa_K2885000:Design

Designed by: Sungho Ko   Group: iGEM18_BioMarvel   (2018-09-17)


Protien G (ProG)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 430


Design Notes

Sequence was synthesized using gBlocks Gene Fragment, and it went through a codon optimization process to eliminate the complexity of the sequence.


Source

The DNA fragments encoding ProG were amplified by the genomic DNA of Staphylococcus aureus Mu50 as a template.

References